Product
AminoSupplement
Ajinomoto CELLiST Korea’s CELLiST™ Amino Supplement Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST™ Media are high-performance, chemically-defined media, free from any animal-derived ingredients.
By using media supplements for cell culture experiments, there are many advantages for titer improvement as well as cost effectiveness. CELLiST™ AminoSupplement help ensure the reliability and consistency of your cell culture and research results.
| Product name | Main Target | Format | Package type | Qty. |
|---|---|---|---|---|
| AminoSupplement Cys1 | Single-agent feed media users | Powder | 1 L Aluminum pouch | 6.61 g |
| 50 L Plastic Drum | 331 g | |||
| AminoSupplement Cys2 | Two-agent feed media users | Powder | 1 L Aluminum pouch | 17.0 g |
| 25 L Plastic Drum | 425 g |
Titer improvement
Adding AminoSupplement Cys1/2 increase Cysteine availability in the media by inhibiting Cystine formation. This leads to improvement in overall productivity, as can be seen below.
CHO-DG44/ambr®
Fed-batch cultures were compared using cells of CHO-DG44. FEED2 was added 5.25% on days 4, 6, 8, 10, and 12 of culture.
CHO-S/ambr®
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 6% to the basic media from other companies on culture days 4, 6, 8, 10, and 12.
CHO-S/ambr®
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 4.5% to the basic media from other companies on cultures 4, 6, 8, 10, and 12 days.
Effect on the culture process
Adding AminoSupplement Cys1/Cys2 has no major impact on glycan profile and Charge Variant as follows, so you can use it without any significant change in the culture process.
Glycan profile
Charge Variant
Costs-cutting by improved stability [AminoSupplement Cys2]
Two-agent feed media requires two tanks due to instability. However, adding AminoSupplement Cys2 improves the stability, which allows it to be prepared in a single tank and save much initial & running costs.
| day | 0 | 10 | 14 | 17 | 21 | 24 | 28 | 31 | |
| Other feed media(feed A+B) w/o AminoSupplement Cys2 |
10 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
|---|---|---|---|---|---|---|---|---|---|
| 40 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| Other feed media(feed A) w/ AminoSupplement Cys2 |
10 mL | — | — | — | — | — | — | — | + |
| 40 mL | — | — | — | — | — | — | — | — |
- : No precipitation + : Slightly precipitated ++ : Clearly precipitate +++ : Large amounts of precipitates
