CELLiST
 

Product

AminoSupplement

Ajinomoto CELLiST Korea’s CELLiST Amino Supplement Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

Improve your cell culture with CELLiST AminoSupplement

By using media supplements for cell culture experiments, there are many advantages for titer improvement as well as cost effectiveness. CELLiST AminoSupplement help ensure the reliability and consistency of your cell culture and research results.

Features
  • Chemically defined feed media supplement
  • Suitable for all types of feed media
  • Packaging form can be adjusted according to customer’s requirements
  • Does not contain :
    - Does not contain animal-derived components
    - Does not contain hydrolysates, extracts or other undefined components
    - Does not contain protein like growth factors
    - Does not contain L-glutamine sources, sodium bicarbonate, or poloxamer

Specifications

Product name Main Target Format Package type Qty.
AminoSupplement Cys1 Single-agent feed media users Powder 1 L Aluminum pouch 6.61 g
50 L Plastic Drum 331 g
AminoSupplement Cys2 Two-agent feed media users Powder 1 L Aluminum pouch 17.0 g
25 L Plastic Drum 425 g

Performance

Titer improvement

Adding AminoSupplement Cys1/2 increase Cysteine availability in the media by inhibiting Cystine formation. This leads to improvement in overall productivity, as can be seen below.

CHO-DG44/ambr®

Fed-batch cultures were compared using cells of CHO-DG44. FEED2 was added 5.25% on days 4, 6, 8, 10, and 12 of culture.

CHO-S/ambr®

Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 6% to the basic media from other companies on culture days 4, 6, 8, 10, and 12.

CHO-S/ambr®

Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 4.5% to the basic media from other companies on cultures 4, 6, 8, 10, and 12 days.

Effect on the culture process

Adding AminoSupplement Cys1/Cys2 has no major impact on glycan profile and Charge Variant as follows, so you can use it without any significant change in the culture process.

Glycan profile

Charge Variant

Costs-cutting by improved stability [AminoSupplement Cys2]

Two-agent feed media requires two tanks due to instability. However, adding AminoSupplement Cys2 improves the stability, which allows it to be prepared in a single tank and save much initial & running costs.

day 0 10 14 17 21 24 28 31
Other feed media(feed A+B)
w/o AminoSupplement Cys2
10 mL +++ +++ +++ +++ +++ +++ +++ +++
40 mL +++ +++ +++ +++ +++ +++ +++ +++
Other feed media(feed A)
w/ AminoSupplement Cys2
10 mL +
40 mL

- : No precipitation     + : Slightly precipitated     ++ : Clearly precipitate     +++ : Large amounts of precipitates

SAMPLE
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