BASAL
CHO MX

Product Basal Media

CELL CULTURE MEDIA

Ajinomoto CELLiST Korea’s CELLiST BASAL Media for Chinese Hamster Ovary (CHO) cell lines are developed based on Ajinomoto’s strong capabilities in amino acid technology and over 30 years of experience in the cell culture media business. CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

BASAL CHO MX

growth medium provides optimal balance of amino acids and other nutrients to ensure adequate cell growth and maximum productivity of your process. BASAL CHO MX Medium is completely chemically-defined and does not contain any animal or plant-derived components.

  • Does not contain thymidine or hypoxanthine
  • Does not contain L-glutamine source
  • Does not contain sodium bicarbonate
  • Contains 6.2 g/L Glucose
  • Contains Poloxamer
Properties
  • Chemically-defined, protein-free medium without any animal-derived components, hydrolysates, extracts or other undefined components
  • Suitable for all CHO cell lines including CHO-M, CHO-GS, CHO-K1, CHO-S, and CHO-DG44
  • Suitable for batch, fed-batch, and perfusion cell cultures, at any scale
  • High performance in both cell growth and protein production
  • Test samples as well as bulk size orders are available
  • Flexible application for easily replacing any existing media platform
  • Manufactured in a cGMP-complied factory

Specifications

Model No. BASAL CHO MX
TYPE Powder
Concentration 23.0g / L
With Additives D-Glucose · Poloxamer
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 24 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

Below are cell culture performance results for CELLiST BASAL CHO MX Medium and several other commer- cially-available media. The fed-batch process was performed using ambr15.

Cell Growth

Figure 1. Viable cell density profiles during 14 days of culture.

Protein Production

Figure 2. IgG titer profile. IgG concentration was measured by Cedex Bio HT.

Scalability
As can be seen below, CELLiST BASAL CHO MX Medium is suitable for use from small-scale to larger scale 200L bioreactors.

Figure 3. Viable cell density and IgG titer profiles. 3 kinds of bioreactor were used, which are ambr15, 3L bench-top reactor, and 200L bioreactor.

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