Product Feed Media
CELL CULTURE FEED MEDIA
Ajinomoto CELLiST Korea’s CELLiST™ FEED Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST™ Media are high-performance, chemically-defined media, free from any animal-derived ingredients.
CELLiST™ FEED Media is a highly concentrated feed medium designed for achieving optimal culture performance in CHO cell lines. CELLiST™ FEED Media will ensure optimal culture growth and productivity, as well as protein quality, to satisfy your process goals. CELLiST™ FEED Media could be used in combination with any media, although designed and optimized for use with CELLiST™ BASAL Media.
CELLiST™ F7 feed medium employs Ajinomoto Group’s proprietary cysteine-stabilization technology, ensuring high concentrations of cysteine are continuously available for your CHO cells throughout the culture, leading to greatly improved performance. This is achieved by drastically reducing the naturally-occurring oxidation of L-cysteine to cystine dimers, leading to increase in the amounts of available cysteine, while also improving stability and shelf-life.
CELLiST F7
IgG Titer
Above figure shows >20% increase in performance following 50% increase in available cysteine (1x compared to 1.5x)
(ambr15® culture data, CHO-K1 cell line)
Optimization of amino acids formulation through joint development
with A*STAR’s Bioprocess Technology Institute.
Cutting-edge ‘Digital Twin’ and AI technologies were employed for optimizing metabolic flux in key pathways, such as the TCA cycle, leading to significant improvement in specific productivity and culture growth, and reduced levels of toxic by-products.
| Model No. | F7 |
|---|---|
| TYPE | Powder |
| Concentration | 120.0g / L |
| With Additives | None |
| Without Additives | L-Glutamine·D-Glucose·Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 24 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to five popular commercial media combinations in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
CHO-GS cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with F7
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.
