F7
 

Product Feed Media

CELL CULTURE FEED MEDIA

Ajinomoto CELLiST Korea’s CELLiST FEED Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

CELLiST FEED Media

CELLiST FEED Media is a highly concentrated feed medium designed for achieving optimal culture performance in CHO cell lines. CELLiST FEED Media will ensure optimal culture growth and productivity, as well as protein quality, to satisfy your process goals. CELLiST FEED Media could be used in combination with any media, although designed and optimized for use with CELLiST BASAL Media.

Features
  • Enhanced titer and stability by utilizing Cysteine stabilization technology
  • Reduced Lactate and increased titer by optimizing amino acids formulation
  • Process simplification using single-agent feed process
  • Chemically formulated and free of animal-derived ingredients
  • Contains high-quality amino acids based on Ajinomoto's long-standing expertise in amino acid science
  • Suitable for most CHO cell lines, including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
  • Packaging form can be adjusted according to customer’s needs

Background:

CELLiST F7 feed medium employs Ajinomoto Group’s proprietary cysteine-stabilization technology, ensuring high concentrations of cysteine are continuously available for your CHO cells throughout the culture, leading to greatly improved performance. This is achieved by drastically reducing the naturally-occurring oxidation of L-cysteine to cystine dimers, leading to increase in the amounts of available cysteine, while also improving stability and shelf-life.

CELLiST F7

IgG Titer

Above figure shows >20% increase in performance following 50% increase in available cysteine (1x compared to 1.5x)
(ambr15® culture data, CHO-K1 cell line)

Optimization of amino acids formulation through joint development
with A*STAR’s Bioprocess Technology Institute.

Cutting-edge ‘Digital Twin’ and AI technologies were employed for optimizing metabolic flux in key pathways, such as the TCA cycle, leading to significant improvement in specific productivity and culture growth, and reduced levels of toxic by-products.

Specifications

Model No. F7
TYPE Powder
Concentration 120.0g / L
With Additives None
Without Additives L-Glutamine·D-Glucose·Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 24 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to five popular commercial media combinations in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-GS cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

SAMPLE
REQUEST
Logo

Start typing and press enter!