Product Feed Media
CELL CULTURE FEED MEDIA
Ajinomoto CELLiST Korea’s CELLiST™ FEED Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST™ Media are high-performance, chemically-defined media, free from any animal-derived ingredients.
CELLiST™ FEED Media is a highly concentrated feed medium designed for achieving optimal culture performance in CHO cell lines. CELLiST™ FEED Media will ensure optimal culture growth and productivity, as well as protein quality, to satisfy your process goals. CELLiST™ FEED Media could be used in combination with any media, although designed and optimized for use with CELLiST™ BASAL Media.
| Model No. | FEED2 |
|---|---|
| TYPE | Powder |
| Concentration | 110.0g / L |
| With Additives | None |
| Without Additives | L-Glutamine·D-Glucose·Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 36 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 6% of FEED2 was added on days 4, 6, 8, 10 and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-GS (CHOZN®) cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against six popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 was evaluated against eight popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-S cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 6, 8, and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
