FEED2
 

Product Feed Media

CELL CULTURE FEED MEDIA

Ajinomoto CELLiST Korea’s CELLiST FEED Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

CELLiST FEED Media

CELLiST FEED Media is a highly concentrated feed medium designed for achieving optimal culture performance in CHO cell lines. CELLiST FEED Media will ensure optimal culture growth and productivity, as well as protein quality, to satisfy your process goals. CELLiST FEED Media could be used in combination with any media, although designed and optimized for use with CELLiST BASAL Media.

Features
  • Chemically formulated and free of animal-derived ingredients
  • Contains high-quality amino acids based on Ajinomoto's long-standing expertise in amino acid science
  • Suitable for most CHO cell lines, including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
  • Packaging form can be adjusted according to customer’s needs

Specifications

Model No. FEED2
TYPE Powder
Concentration 110.0g / L
With Additives None
Without Additives L-Glutamine·D-Glucose·Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 6% of FEED2 was added on days 4, 6, 8, 10 and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-GS (CHOZN®) cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against six popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 was evaluated against eight popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-S cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 6, 8, and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

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