BASAL3/3P
 

Product Basal Media

CELL CULTURE MEDIA

Ajinomoto CELLiST Korea’s CELLiST BASAL Media for Chinese Hamster Ovary (CHO) cell lines are developed based on Ajinomoto’s strong capabilities in amino acid technology and over 30 years of experience in the cell culture media business. CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

CELLiST BASAL Media

CELLiST BASAL Media are all Chemically Defined Media, optimized for Chinese Hamster Ovary (CHO) cells cultures and the production of recombinant proteins. CELLiST BASAL Media are developed based on Ajinomoto's capabilities in cell culture media development and know-how of amino acids science. CELLiST BASAL Media can be applied to various CHO cell lines, including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.

Features
  • Chemically formulated and free from animal-derived ingredients
  • Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise
  • Suitable for most CHO cell lines including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
  • Packaging form can be adjusted according to customer’s requirements

Specifications

Model No. BASAL3
TYPE Powder
Concentration 27.0g / L
With Additives D-Glucose
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Model No. BASAL3P
TYPE Powder
Concentration 28.0g / L
With Additives D-Glucose · Poloxamer
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine· Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 24 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with CELLiST F7

A Viable cell density

B IgG Titer (Day 14)

Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11. ‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.

Protein Quality Across Different Scales

Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system, and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.

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