F7
 

Product Feed Media

细胞培养用补料培养基

基于Ajinomoto(株)的氨基酸技术和三十年以上对动物细胞培养基生产领域的经验而开发的动物细胞培养基。 生产和销售以化学定义成分的不含动物源的高性能培养基。

CELLiST FEED Media

是被设计成可提高CHO细胞株的培养性能的高浓缩补料培养基。 CELLiSTFEED Media在保持蛋白质品质和功能的同时,支持完成所追求的细胞成长和生产效率。 FEED2可以和所有培养基一起使用,但是基本上最适合同CELLiST BASAL Media一起使用。

Features
  • 利用半胱氨酸稳定技术提高效力和稳定性
  • 优化的氨基酸配方可减少乳酸并提高效价
  • 通过单一代理供应流程简化您的流程
  • 由化学成分组成,不含动物源成分
  • 含有以Ajinomoto长期积累的氨基酸研究实力及专业知识为基础进行配制的优质氨基酸
  • 适合于包括CHO-K1、CHO-GS、CHO-S、CHO-DG44及CHO-DXB11在内几乎所有的CHO细胞系
  • 可根据客户要求提供多种形式的包装规格

Background:

CELLiST F7 feed medium employs Ajinomoto Group’s proprietary cysteine-stabilization technology, ensuring high concentrations of cysteine are continuously available for your CHO cells throughout the culture, leading to greatly improved performance. This is achieved by drastically reducing the naturally-occurring oxidation of L-cysteine to cystine dimers, leading to increase in the amounts of available cysteine, while also improving stability and shelf-life.

CELLiST F7

IgG Titer

Above figure shows >20% increase in performance following 50% increase in available cysteine (1x compared to 1.5x)
(ambr15® culture data, CHO-K1 cell line)

Optimization of amino acids formulation through joint development
with A*STAR’s Bioprocess Technology Institute.

Cutting-edge ‘Digital Twin’ and AI technologies were employed for optimizing metabolic flux in key pathways, such as the TCA cycle, leading to significant improvement in specific productivity and culture growth, and reduced levels of toxic by-products.

Specifications

Model No. F7
TYPE Powder
Concentration 120.0g / L
With Additives None
Without Additives L-Glutamine·D-Glucose·Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 24 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to five popular commercial media combinations in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-GS cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

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