BASAL3/3P
 

Product Basal Media

细胞培养基

基于Ajinomoto(株)的氨基酸技术和三十年以上对动物细胞培养基生产领域的经验成功开发的动物细胞培养基。 生产和销售以化学定义成分的不含动物源的高性能培养基。

CELLiST BASAL Media

是最适合于Chinese Hamster Ovary (CHO)细胞成长和重组蛋白质的化学定义成分的基础培养基。 CELLiST BASAL Media是基于Ajinomoto的实力及对氨基酸的经验进行开发的,可适用于CHO-K1、CHO-S、CHO-DG44、CHO-DXB11等多种CHO细胞系。

特征
  • 由化学成分组成,不含动物源成分
  • 包含着基于Ajinomoto长期的氨基酸研究能力和专业知识进行配制的优质氨基酸
  • 适合于包括CHO-K1、CHO-GS、CHO-S、CHO-DG44及CHO-DXB11在内几乎所有CHO细胞系
  • 可根据客户要求提供多种形式的包装规格

Specifications

Model No. BASAL3
TYPE Powder
Concentration 27.0g / L
With Additives D-Glucose
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Model No. BASAL3P
TYPE Powder
Concentration 28.0g / L
With Additives D-Glucose · Poloxamer
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine· Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 24 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with CELLiST F7

A Viable cell density

B IgG Titer (Day 14)

Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11. ‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.

Protein Quality Across Different Scales

Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system, and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.

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