Product Basal Media
细胞培养基
基于Ajinomoto（株）的氨基酸技术和三十年以上对动物细胞培养基生产领域的经验成功开发的动物细胞培养基。 生产和销售以化学定义成分的不含动物源的高性能培养基。
是最适合于Chinese Hamster Ovary (CHO)细胞成长和重组蛋白质的化学定义成分的基础培养基。 CELLiST™ BASAL Media是基于Ajinomoto的实力及对氨基酸的经验进行开发的，可适用于CHO-K1、CHO-S、CHO-DG44、CHO-DXB11等多种CHO细胞系。
| Model No. | BASAL3 |
|---|---|
| TYPE | Powder |
| Concentration | 27.0g / L |
| With Additives | D-Glucose |
| Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 36 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
| Model No. | BASAL3P |
|---|---|
| TYPE | Powder |
| Concentration | 28.0g / L |
| With Additives | D-Glucose · Poloxamer |
| Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine· Insulin and other growth factors |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 24 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with CELLiST F7
A Viable cell density
B IgG Titer (Day 14)
Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11. ‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.
Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system, and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.
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