Product Basal Media
细胞培养基
Ajinomoto CELLiST Korea生产的用于CHO细胞系的CELLiST™基础培养基是基于味之素在氨基酸技术方面的强大能力和30多年的细胞培养基业务经验而开发的。 CELLiST™培养基是高性能、化学成分明确的培养基,不含任何动物源性成分。
培养基提供氨基酸和其他营养物质的最佳平衡,以确保细胞充分地生长和工艺效率最大化。BASAL CHO MX培养基是完全化学定义的,不含任何动物来源的成分。
Model No. | BASAL CHO MX |
---|---|
TYPE | Powder |
Concentration | 23.0g / L |
With Additives | D-Glucose · Poloxamer |
Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Insulin and other growth factors |
Storage Condition | 2℃ to 8℃, dark and dry |
Shelf Life | 24 months |
Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
Below are cell culture performance results for CELLiST BASAL CHO MX Medium and several other commer- cially-available media. The fed-batch process was performed using ambr15.
Cell Growth
Figure 1. Viable cell density profiles during 14 days of culture.
Protein Production
Figure 2. IgG titer profile. IgG concentration was measured by Cedex Bio HT.
Scalability
As can be seen below, CELLiST BASAL CHO MX Medium is suitable for use from small-scale to larger scale 200L bioreactors.
Figure 3. Viable cell density and IgG titer profiles. 3 kinds of bioreactor were used, which are ambr15, 3L bench-top reactor, and 200L bioreactor.