F7
 

Product Feed Media

세포배양배지

아지노모도㈜의 아미노산 기술과 30년이상 동물세포배양배지사업에 대한 경험을 바탕으로 개발된 동물세포배양배지입니다. 최근엔 모두 Chemically Defined 성분으로 동물유래성분을 포함하지 않은 고성능 배지를 제조·판매하고 있습니다.

CELLiST FEED Media

CHO 세포주의 배양 성능을 향상시킬 수 있도록 설계된 고농축 Feed 배지입니다. CELLiST FEED Media는 단백질 품질과 기능을 유지하면서 목표하는 세포 성장과 생산성을 달성할 수 있도록 지원합니다. FEED2는 모든 배지와 함께 사용할 수 있지만, 기본적으로는 CELLiST BASAL Media와 함께 사용하는데 최적화되었습니다.

특징
  • Cysteine 안정화 기술을 활용하여 역가 및 안정성 향상
  • 아미노산 제형 최적화로 젖산 감소 및 역가 증가
  • 단일 에이전트 공급 프로세스를 사용한 프로세스 단순화
  • 화학적으로 조성되었으며 동물 유래 성분이 없음
  • 아지노모도의 오랜 아미노산 연구 역량 및 전문 지식을 바탕으로 배합한 고품질의 아미노산들을 포함하고 있음
  • CHO-K1, CHO-GS, CHO-S, CHO-DG44 및 CHO-DXB11 을 포함한 거의 모든 CHO 셀 라인에 적합함
  • 다양한 사용 목적에 적합한 형태로 포장의 변형이 가능

Background:

CELLiST F7 feed medium employs Ajinomoto Group’s proprietary cysteine-stabilization technology, ensuring high concentrations of cysteine are continuously available for your CHO cells throughout the culture, leading to greatly improved performance. This is achieved by drastically reducing the naturally-occurring oxidation of L-cysteine to cystine dimers, leading to increase in the amounts of available cysteine, while also improving stability and shelf-life.

CELLiST F7

IgG Titer

Above figure shows >20% increase in performance following 50% increase in available cysteine (1x compared to 1.5x)
(ambr15® culture data, CHO-K1 cell line)

Optimization of amino acids formulation through joint development
with A*STAR’s Bioprocess Technology Institute.

Cutting-edge ‘Digital Twin’ and AI technologies were employed for optimizing metabolic flux in key pathways, such as the TCA cycle, leading to significant improvement in specific productivity and culture growth, and reduced levels of toxic by-products.

Specifications

Model No. F7
TYPE Powder
Concentration 120.0g / L
With Additives None
Without Additives L-Glutamine·D-Glucose·Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Related Documents

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to five popular commercial media combinations in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-GS cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with F7

A Viable cell density

B IgG Titer (Day 14)

CELLiST BASAL3+F7 was compared to another commercial media combination in a fed-batch culture. From days 3 to 11, 6% of feeding was done according to each manufacturer’s protocol.

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